08
2017
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11
Purification strategy for swine fever and swine pseudorabies in breeding farms
Swine fever (CSF) and porcine pseudorabies (PR) are two important infectious diseases of pigs, which have caused great economic losses to my country's pig industry. It threatens the health of pig herds. Practice has proved that pig herds in my country carry CSFV and PRV wild viruses to varying degrees, which often lead to persistent infection, invisible virus carriers, and complicated and difficult-to-treat pig diseases. Therefore, the fundamental measure is to purify each pig herd (especially the core breeding herd), and adopt the strategy of immunization + detection + grouping + elimination, which is an ideal way for breeding pig farms to eradicate CSFV and PRV.
1 Epidemiological investigation Carry out epidemiological investigations such as CSF, PR, PRRS, etc., to grasp the overall status of the pig herd, to clarify the background information of the epidemic disease in the breeding farm, and to provide a basis for purification.
2. Immunization program According to the results of epidemiological investigations, disease immunization programs such as CSF and PR are formulated. Refer to the immunization program (omitted).
3. Vaccine selection Select the vaccine from a regular and legal veterinary vaccine production unit. For example, the swine fever heat-resistant protective agent live vaccine (rabbit source), the swine fever live vaccine (spleen lymphocyte source), the swine fever live vaccine (cell source) and the pseudorabies live vaccine (PRV gE deletion live vaccine) produced by Zhongbo Company, according to Instructions for correct use.
4. Sample collection Samples were collected 4 weeks after vaccination, and pig tonsils, nasal cotton swabs, blood, etc. were collected from each tested pig for simultaneous testing. The first sampling of reserved young pigs, multiparous sows, adult boars, and introduced reserve breeding pigs must be carried out one by one, and then sampling at a rate of 10%-30%. Piglets and nursery pigs are sampled at a rate of 5-10%. After the samples are processed and distributed, they are stored at low temperature.
5 Detection methods Pig tonsil samples were detected by fluorescence quantitative PCR (FQ-PCR), RT-PCR, immunofluorescence (IFA) and other methods to detect wild CSFV virus, while tonsil and nasal cotton swab samples were detected by PCR method for wild PRV virus. Pig serum was detected by IDEXX kits for CSFV immune antibodies, gB-ELISA kits for PRV immune antibodies, and gE-ELISA kits for PRV infectious antibodies.
6 Results and treatment If any of the CSFV and PRV pathogen detection methods and PRV gE-ELISA antibody test are positive, the tested pigs are determined to be carrying CSFV/PRV wild virus or infected with PRV wild virus, and all tested positive pigs should be grouped and isolated. Feeding, immediate culling if both methods were positive, retesting if one method tested positive, and culling if positive again. The detection of CSFV and PRV immune antibodies is carried out according to their respective kits, and the breeding pigs that fail to pass the multiple detections of CSFV or PRV immune antibodies and have poor production performance will be eliminated.
7 Purification standards are tested 2-3 times a year. The positive rate (or wild virus infection rate) of CSFV and PRV in each pig herd must be less than 1%. When other pigs reach more than 85%, it is basically up to the standard. After 2-3 years of repeated testing and elimination treatment, the breeding farm establishes a completely healthy CSFV and PRV negative breeding herd.
8. Other breeding farms implement a multi-point feeding model, strengthen the awareness of biosecurity, insist on self-propagation and self-support, and strictly introduce and disinfect and isolate. The agricultural sector should strengthen epidemic early warning and monitoring, strictly quarantine, increase funding, and further improve the epidemic prevention and control system.
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